Animal and Plant-based Extenders for Liquid Storage of Tom Semen Preserved at 5oC
Adedeji Suleimon Balogun1*, Akintunde Akinbola Akinosun1, Adetoun Janet Ajibade2, Taiwo Hazzan Ojetunde1, Bisola Barakat Hammed1
1Department of Animal Production Technology, Oyo State College of Agriculture and Technology, Igboora.Oyo State. Nigeria.
2Department of Agricultural Technology, Oyo State College of Agriculture and Technology, Igboora.Oyo State. Nigeria.
Abstract
The objective was to compare fertility of turkey tom semen extended in Tris coconut-water orange juice (TCWO) or Tris Egg-yolk Orange Juice (TEYO) and stored at 5oC for 4 or 12 h. Pooled ejaculates from five toms were divided into five portions; one portion was not extended (Control; inseminated immediately); two portions were extended in TCWO and the remaining two in TEYO. Extended semen was either inseminated immediately or kept in a refrigerator (5oC) for 4 h (Chilled) prior to insemination. Hens (n=45) were allocated into five groups and inseminated once weekly (with ~200 x 106 viable sperm) for 4wk with 0.05 mL of un-extended, TCWO, TEYO extended semen and 0.1 mL of 4h TCWO, TEYO Chilled semen. The experiment was subsequently repeated with 12 h chilled TCWO and TEYO semen. Eggs were collected daily, stored at room temperature and incubated weekly. The fertility rate (fertile/incubated eggs×100) was determined by candling 21 d after the start of incubation date were analyzed by one-way ANOVA and differences located with DMRT. Fertility and hatchability rate were significantly different (P<0.05) among Control, extended semen (TCWO vs TEYO) and chilled semen (TCWO vs TEYO) for both 4h and 12h cold storage preservation. Although 4h and 12h TCWO chilled tom semen had better values for hatchability of fertile eggs and eggs set. TCWO extender improved Fertility or hatchability for extended semen compared to TEYO extender as well as better fertility and hatchability was observed for the chilled semen compared to TEYO extender.
Keywords: Animal, Plant, Extender, Semen, Fertility, Hatchability
Full length article *Corresponding Author, e-mail: balogunadedeji001@gmail.com, doi# 10.71111/Science today/5-25-2-2-st-5